Figure 9. Lymphomas from individual aged vPK mice are monoclonal or polyclonal.
D–JH segments of IgH in genomic DNA were PCR amplified, electrophoresed, and stained with ethidium bromide. D–JH segments of IgH in normal WT spleens (WT Sp) and vPK lymphomas. Node, mouse 3; liver, mice 4, 7, and 13; spleen, mice 6, 8–12, and 14. Asterisks indicate the preferential amplification of 1 D–JH segment. A total of 20 lymphoma tissues each from a different aged vPK mouse were evaluated. Polyclonal, n = 12; monoclonal, n = 8.