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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Nat Neurosci. 2017 Nov 27;21(1):29–32. doi: 10.1038/s41593-017-0023-y

Fig. 2. Anatomical evidence for a direct orexinLH→D2NAc circuit.

Fig. 2

A. Left: targeting strategy. Right: eGFP expression in D2NAc shell neurons, zoomed images are included to confirm labelling of cell bodies in NAc shell rather than core (representative example of n = 4 brains).

B. Left histology images: Orexin and MCH immunoreactivity in LH neurons that directly innervate D2NAc cells, representative examples from n = 4 brains. Right plot: incidence of orexin and MCH immunoreactivities D2NAc -projecting LH neurons (values are mean±sem and raw values for individual brains). Number above bars is p value from unpaired t-test with Welch’s correction, t,df = 8.611,4.723 (analysis of 1636 cells from 4 brains).