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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2018 Feb 5;29(5):859–865. doi: 10.1007/s13361-017-1880-z

Figure 1.

Figure 1

PepSAVI-MS pipeline modifications for secreted peptides. Modifications to the originally proposed PepSAVI-MS pipeline for (a) collection of secreted peptides, and (b) creation of fraction libraries and addition of each library to the adapted bioassay screen against relevant fungal species. Harvesting of secreted peptides includes large-culture microbial growth, secretome isolation via centrifugation, addition of weak cation exchange peptide binding resin to cell-free supernatent, and elution of peptides off of the resin. Eluted peptides are then fractionated into libraries that are subject to bioactivity screening via agar diffusion activity assays