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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: ACS Nano. 2017 Nov 1;11(11):10712–10723. doi: 10.1021/acsnano.7b04878

Figure 1.

Figure 1

Schematic illustration of the ExoTIC device for extracellular vesicle isolation. (a) Various biofluids can be processed for EV isolation including culture media, plasma, and urine. (b) Schematic illustration of size-based EV isolation using the ExoTIC device. Intact EVs are enriched and purified at the filter, whereas the free proteins and nucleic acids are washed out. (c) Downstream analysis of EVs isolated from different clinical sample types for size, morphology, and molecular contents. (d) Schematic process of EV isolation from sample-in to EV-out. Device operation includes isolation of EVs from cell culture media (5 mL in 1 h), washing with PBS buffer (5 mL in 1 h), and collection of ~200 μL of EV solution for subsequent analysis. Total operation time for 5–10 mL of sample is under 3 h.