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Panel a, the docked state populations found from the FMN fluorescence lifetime measurements. Panel b, docked state stabilization energies (relative to kT at room temperature) estimated using Eq. 3 from the Pd values shown in panel a. The Pd value at I = 0 mM shown by the open circle in panel a was reported in ref. 13, and the corresponding Ed value (open circle in panel b) was estimated in this work.
