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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Rep. 2018 May 8;23(6):1817–1830. doi: 10.1016/j.celrep.2018.04.026

Figure 6. AAP Promotes VP-VP Interactions.

Figure 6

(A) Schematic of expression constructs for AAP2 and VP1 and VP3 of AAV2, AAV3, Anc82, and 82DI. In CMV-HA-VP1, the VP2 and VP3 start codons were modified to silence their expression, and the AAPstop60 mutation (red rectangle) was included.

(B) HEK293 cells were transfected with CMV-HA-VP1 and CMV-VP3 of the serotype indicated above each lane, with or without CMV-AAP2, and lysates were harvested after 48 hr. IP was performed using anti-HA antibody. VPs were detected by western blot using the B1 antibody.

(C) Lysates from CMV-HA-VP1 and CMV-VP3, with or without CMV-AAP2-transfected (all AAV2 proteins) HEK293 cells, were treated with DMSO, 5 mM disuccinimidyl glutarate (DSG), or 5 mM disuccinimidyl suberate (DSS), as indicated above the columns. VPs were detected by western blot with B1 antibody. Approximate molecular weights are shown at the right of each row.

See also Figure S7.