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d17dhSph dose-dependent label incorporation. MCF-7 cells were labeled with 50, 100, 500, or 1,000 nM of 17 carbon backbone dihydrosphingosine (precursor) (17CdhSph) for a 120 min pulse, then harvested and analyzed with HPLC/MS/MS. Intracellular levels of d17dhSph (A), dhCer d17:0/16:0 (B), d17Cer (C), d17SM (50 nM not acquired) (D), and d17HexCer (E) are presented (n = 2). Data are expressed as mean ± SD.
