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Time-dependent incorporation of d17dhSph label into sphingoid base and phosphate. MCF-7 cells were labeled with 500 nM d17dhSph. Cells were harvested at relevant time points and analyzed with HPLC/MS/MS. A: d17dhSph-l-P levels during entire 120 min pulse. B: d17Sph levels throughout the full 120 min pulse. Data are expressed as mean ± SD, n = 3.
