TABLE 1.

In situ sphingolipid enzymatic activity rates

Phase	Vehicle (pmol/nmol Pi/min)	FB1 (pmol/nmol Pi/min)	GT-11 (pmol/nmol Pi/min)	BFA (pmol/nmol Pi/min)
CerS (total)	0.61 (100%)	0.061 (10%)	0.60 (98%)	0.54 (89%)
DeS	0.85 (100%)	NI	0.026 (3%)	0.24 (28%)
HexCer synthesis	0.37 (100%)	NI	NI	0.39 (105%)
SM synthesis	0.39 (100%)	NI	NI	1.2a (308%)

Rates are the calculated as slope of label incorporation into the product analyte for a given enzymatic reaction during the time frames (phases) established in Figs. 4–6. Units are picomolesof labeled product per nanomole of lipid Pi per minute. Percent vehicle is also given. NI, no incorporation.

^aRate for adjusted phase.