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. 2018 Apr 20;59(6):994–1004. doi: 10.1194/jlr.M082891

Fig. 3.

Fig. 3.

TgACS-HA-iKO is a dispensable cytosolic protein that can be downregulated to determine its cellular function. A: IFA images of TgACS-HA-iKO strain with or without ATc. TgACS-HA fluorescent signal is shown in green. DAPI, as a marker for the nucleus, is shown in blue. IMC1 and TOM40 are markers of the inner membrane complex and the parasite mitochondria, respectively, and both are shown in red. TgACS-HA localizes in the cytosol, and this signal is lost after the addition of ATc. B: Western blotting image of TgACS-HA-iKO strain. TgACS-HA protein is detected at the predicted molecular mass, 79.8 kDa. The addition of ATc causes complete protein loss after 3 days. C: Plaque assays were used to evaluate the growth of the TgACS-HA_iKO strains with or without ATc in comparison to T. gondii reference strain, Tati-Ku80. D: Statistical analysis of plaque assay was performed to show that there was no difference in the presence or absence of TgACS protein. All scale bars, 5 μm.