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Wild-type (wt) cells and ESCRT mutant cells grown at log phase were spotted onto plates containing YPDA or YPDA plus 4.5mM LiCl, 4mM CoCl2, 650mM MgCl2, 1.5M KCl, 350mM CaCl2, 3mM NiSO4 and 6 mM MnCl2, respectively, and then incubated at 27°C for 4 days.
