Table 1. S. pombe genes identified in NaCl -resistant screen.
| Category and systematic gene name |
Common gene name |
Gene description | NaCl resistance |
|---|---|---|---|
| Membrane trafficking | |||
| SPAC19A8.05c | sst4 | ESCRT 0 complex subunit Sst4, sorting receptor for ubiquitinated membrane proteins | +++ |
| SPBC651.05c | dot2 | ESCRT II complex subunit Dot2 | +++ |
| SPBC4B4.06 | vps25 | ESCRT II complex subunit Vps25 | +++ |
| SPBC3B9.09 | vps36 | ESCRT II complex subunit Vps36 | +++ |
| SPBC215.14c | vps20 | ESCRT III complex subunit Vps20 | +++ |
| SPAC4F8.01 | did4 | ESCRT III complex subunit Did4 | +++ |
| SPAC1142.07c | vps32 | ESCRT III complex subunit Vps32 | +++ |
| SPAC17G6.05c | bro1 | BRO1 domain protein Bro1 | +++ |
| SPBC8D2.02c | vps68 | vacuolar sorting protein Vps68 | ++ |
| SPAC17A2.06c | vps8 | CORVET complex WD repeat/ ubiquitin-protein ligase E3 subunit Vps8 | + |
| Regulation of transcription and translation | |||
| SPBC25B2.03a | NA | zf-C3HC4 type zinc finger | +++ |
| SPBC28F2.02 | mep33 | translation machinery associated protein Mep33 | +++ |
| SPBC1861.07 | NA | elongin C (predicted) | ++ |
| SPBC1718.03a | ker1 | DNA-directed RNA polymerase I complex subunit Ker1 | + |
| Ubiquitination | |||
| SPBC31F10.10ca | NA | zf-MYND type zinc finger protein | +++ |
| SPBC1105.09 | ubc15 | ubiquitin conjugating enzyme E2 Ubc15 | + |
| SPBC16G5.03 | NA | ubiquitin-protein ligase E3 | + |
| Nucleic acid metabolism | |||
| SPAC4G9.11c | cmb1 | cytosine-mismatch binding protein 1 | +++ |
| SPAC30D11.07 | nth1 | DNA endonuclease III | ++ |
| Amino acid synthesis and metabolism | |||
| SPAC22A12.06c | fsh2 | serine hydrolase-like | ++ |
| SPAC4G9.10 | arg3 | ornithine carbamoyltransferase Arg3 | + |
| Ribosome biogenesis and assembly | |||
| SPAC3F10.17 | ltv1 | ribosome biogenesis protein Ltv1 | ++ |
| SPAC3H5.12c | rpl501 | 60S ribosomal protein L5 | + |
| Histone acetylation and deacetylation | |||
| SPBC16A3.19 | eaf7 | histone acetyltransferase complex subunit Eaf7 | +++ |
| SPAC57A10.14 | sgf11 | SAGA complex subunit Sgf11 | ++ |
| Signal transduction | |||
| SPBC1D7.03a | clg1 | cyclin Clg1 (predicted) | ++ |
| SPAC227.15a | reg1 | protein phosphatase regulatory subunit Reg1 (predicted) |
++ |
| Other functions | |||
| SPBC1778.03c | NA | NADH pyrophosphatase | +++ |
| SPBC660.07 | ntp1 | alpha,alpha-trehalase Ntp1 | +++ |
| SPAC11D3.15 | NA | 5-oxoprolinase (ATP-hydrolizing) | +++ |
| SPAPB1E7.06c | eme1 | Holliday junction resolvase subunit Eme1 | ++ |
| SPAC637.10c | rpn10 | 19S proteasome regulatory subunit Rpn10 | ++ |
| SPAC631.01c | acp2 | F-actin capping protein beta subunit Acp2 | + |
| SPAC15A10.15 | sgo1 | inner centromere protein, shugoshin Sgo2 | + |
a The gene is conserved in fungi only.
Other genes are conserved in both yeast and human. +++, strongly resistant, ++, moderately resistant, +, mildly resistant. NA indicates that common gene name is not applicable. S. pombe gene description was retrieved from the PomBase Database (http://www.pombase.org/).