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. 2018 Jun 1;13(6):e0198639. doi: 10.1371/journal.pone.0198639

Fig 3. Drought and salt stress tolerance of ZmPIP1;1 overexpression transgenic lines.

Fig 3

Five-day-old WT and ZmPIP1;1-Oe transgenic Arabidopsis seedlings were treated with 150 mM Manntiol or 100 mM NaCl in MS medium for 7 days. (A) Photographs of WT and ZmPIP1;1-Oe transgenic Arabidopsis seedlings with 150 mM Manntiol or 100 mM NaCl in MS medium for 7 days. Bar = 1 cm. (B) Primary root length in WT and ZmPIP1;1-Oe with 150 mM Manntiol or 100 mM NaCl in MS medium for 7 days. All data are means of 15 biological replicates with error bars indicating SD, *P<0.01. (C) qRT-PCR analysis of three representative ZmPIP1;1 overexpression transgenic lines. RNA was extracted from the leaves of fourteen-day-old seedlings. All data are means of three biological replicates with error bars indicating SD. Expression of AtACT2 was used as the internal control.