Fig. 2.

Binge drinking of alcohol activates mTORC2 in the DMS. Mice drinking paradigm and dissection scheme are outlined in Fig. 1a. The DMS and DLS were dissected at the end of the last 4 h-binge (B, black) alcohol drinking session. Control animals had access to water only (W, white). a, b Ser⁹GSK3β phosphorylation (pGSK3β) was measured by western blot analysis. GAPDH immunoreactivity was used as an internal loading control. Data are presented as the mean ratio of Ser⁹GSK3β to total GSK3β ± S.E.M, and expressed as percentage of water control. a DMS: t₍₁₅₎ = 3.87, p = 0.0015; n = 8 water, 9 alcohol. b DLS: t₍₇₎ = 0.81, p = 0.444; n = 4 water, 5 alcohol. c, d Ser⁴²²SGK1 phosphorylation (pSGK1) was measured by western blot analysis. GAPDH immunoreactivity was used as an internal loading control. Data are presented as the mean ratio of Ser⁴²²SGK1 to total SGK1 ± S.E.M, and expressed as percentage of water control. c DMS: t₍₁₅₎ = 4.87, p = 0.0002; n = 8 water, 9 alcohol. d DLS: t₍₁₅₎ = 0.31, p = 0.762; n = 8 water, 9 alcohol. **p < 0.01, ***p < 0.001