Figure 8.

Reactive oxygen species (ROS) accumulation and cell death in Atcrk1-1 mutant. (A) ¹O₂ production in leaves of Atcrk1-1 mutant detected by fluorescence of the Singlet Oxygen Sensor Green (SOSG) probe. Plants were infiltrated with SOSG and exposed to high light (10 µM SOSG, 2300 µmole photons m⁻² s⁻¹ for 5 min). Fluorescence was detected by confocal laser scanning microscopy (CLSM). Note intensive fluorescence of SOSG-infiltrated mutant leaves; (B) Cell death in Arabidopsis sp. leaves, detected by Evans blue staining. 3-week old plants were grown either on short day or under continuous light. Note intensive Evans blue reaction in Atcrk1-1 mutants when exposed to continuous light.