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. 2018 May 16;19(5):1476. doi: 10.3390/ijms19051476

Figure 2.

Figure 2

Combined effect of LA and TRAIL on the sub-G1 population and apoptotic proteins in A549 and H1299 non-small cell lung cancer cells. (a) Cells were treated with LA (20 μM) and/or TRAIL (20 ng/mL) for 24 h. The treated cells were fixed with 70% ethanol, stained with propidium iodide (PI) and analyzed by flow cytometry with or without with caspase inhibitors (pan caspase inhibitor; z-VAD-fmk (80 μM), caspase-8 inhibitor; z-IETD-fmk (50 μM)). Bar graphs show quantification of cell cycle population (%). Data represent means ± SD. *** p < 0.001 versus TRAIL alone, # p < 0.05, ### p < 0.001 versus LA+TRAIL treated control. (n = 3)). (b) Cells were treated with LA (20 μM) and/or TRAIL (20 ng/mL) for 24 h. Cell lysates were prepared and subjected to Western blotting for procaspase-8,9,3, Pro-PARP, cleaved caspase-8,9,3, and cleaved-PARP. (c) Cells were treated with LA (20 μM) and/or TRAIL (20 ng/mL) for 24 h. The cells were stained using FITC-Annexin V/PI dye and early and late apoptotic portions were detected by flow cytometry.