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. 2018 May 16;19(5):1476. doi: 10.3390/ijms19051476

Figure 5.

Figure 5

Combined effect of LA and TRAIL on XIAP interaction with caspase3 and NF-κB in A549 non-small cell lung cancer cells. (a) XIAP interacts with caspase3 and NF-κB in the STRING database. Red text (interaction score). (b) A549 cells were treated with LA (20 µM) and/or TRAIL (20 ng/mL) for 24 h. Immunoprecipitation (IP) was performed with lysates from A549 cells using anti-caspase3 and anti-NF-κB antibodies. Western blot analysis was performed to detect XIAP in whole cell lysates. Western blot analysis was performed to detect XIAP, Pro-cas3, and NF-κB in input lysates. Input lysates indicated that the 5% pre-immunoprecipitated samples and β-actin levels were confirmed as being equivalent to protein loading.