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. 2018 May 18;19(5):1507. doi: 10.3390/ijms19051507

Figure 1.

Figure 1

Algorithm of changes in osmotic pressure of culture medium. Osmotic pressure of culture medium was changed or maintained at days 10 and 13. L indicates low-OP; LL and LH indicate continuously low-OP and changed OP from low to high at day 10, respectively; LLL, LHL, and LHH indicate continuously low-OP for 21 days, changed OP from low to high at day 10 followed by changed OP from high to low at day 13, and changed OP from low to high at day 10 followed by continuously high-OP, respectively.