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Immunohistochemistry of spheroids formed with bovine longitudinal depth zone-derived chondrocytes at day 21. Keratan sulfate: KS, collagen type-II: Col-2, collagen type-I: Col-1, and matrix metalloproteinase-13: MMP-13. Brown stain is positive, and brown/dark blue indicates counterstaining of nuclei. Black deposits indicate COL-1, and red indicates counterstaining of nuclei. A bar indicates 100 µm.
