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. 2018 May 9;19(5):1416. doi: 10.3390/ijms19051416

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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3-Methyladenine (3-MA) attenuated the effect of glucosamine (GlcN) on native photoreceptor outer segment (POS)-derived lipofuscin-like autofluorescence (LLAF) in ARPE-19 cells. Cells were treated with GlcN (10 mM), 3-MA (10 mM), native POS, native POS + 3-MA, native POS + GlcN, or GlcN + native POS + 3-MA, and were compared with untreated cells. The mean fluorescence intensity (MFI) was quantified by flow cytometry following seven days of incubation. The results are represented as the mean ± SEM. The differences of the MFI between groups were compared using ANOVA, and Tukey’s test was used for the post hoc analysis; ns, not significant; * p < 0.05 versus the control group; *** p < 0.001 versus the control group; ^# p < 0.05; ^### p < 0.001.