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. 2018 May 11;19(5):1437. doi: 10.3390/ijms19051437

Figure 2.

Figure 2

Rho A is involved in EGF-induced migration of MG63 cells. (A) Western blotting of GTP-Rho A expression in MG63 cells treated with EGF for 2, 4, and 6 h; (B) Quantification of the GTP-Rho A band obtained by western blotting; (C) Western blotting of Rho A and ROCK expression in MG63 cells treated with Rho A shRNA and the mCherry control; (D) Transwell assay of MG63 cells treated with PBS, 10 ng/mL EGF, 10 ng/mL EGF + mCherry, or 10 ng/mL EGF + Rho A shRNA, scale bar = 200 µm; (E) Quantification of invaded MG63 cells treated with PBS, 10 ng/mL EGF, 10 ng/mL EGF + mCherry, or 10 ng/mL EGF + Rho A shRNA; (F) Phalloidin staining of stress fibers and DAPI staining of MG63 cells induced with 10 ng/mL EGF + mCherry or 10 ng/mL EGF + Rho A shRNA, scale bar= 50 μm. * p < 0.05, ** p < 0.01.