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. 2018 May 11;19(5):1437. doi: 10.3390/ijms19051437

Figure 3.

Figure 3

The Rho A inhibitor exoenzyme C3 inhibits EGF-induced MG63 cell invasion and stress fiber formation. (A) Western blotting of GTP-Rho A, Rho A, p-ROCK, and ROCK expression in MG63 cells pretreated with C3 at different concentrations followed by treatment with EGF; (B) Phalloidin staining of stress fibers and DAPI staining of MG63 cells with and without C3 pretreatment followed by the addition of EGF, , scale bar = 50 μm; (C) Results of a wound-healing assay in which MG63 cells with and without C3 pretreatment were treated with EGF for 12, 24, 36 or 48 h; (D) Quantification of migrated MG63 cells with and without C3 pretreatment followed by the addition of EGF for 12, 24, 36 or 48 h, , scale bar = 200 μm * p < 0.05, ** p < 0.01.