ROCK promotes EGF-induced stress fiber formation and cell migration in MG63 cells. (A) Western blotting of phosphorylated ROCK in MG63 cells treated with EGF at 2, 6, and 12 h; (B) Quantification of the ROCK band obtained by western blotting; (C) Phalloidin staining of stress fibers and DAPI staining of MG63 cells pretreated with Y27632 (10 μM) for 2 h followed by the addition of EGF, scale bar = 50 μm; (D) Wound-healing assay of MG63 cells treated with PBS, Y27632, EGF, or EGF + Y27632 for 48 h, scale bar = 200 μm; (E) Quantification of the migrated MG63 cells treated with PBS, Y27632, EGF, or EGF + Y27632 for 48 h. * p < 0.05, ** p < 0.01.