Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 3;19(5):1350. doi: 10.3390/ijms19051350

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Time-dependent effects of MCPIP3 overexpression on protein expressions of epithelial–mesenchymal transition (EMT)-related marker genes in T-REx 293/HA-MCPIP3 cells. (A) Cells were treated with 1 μg/mL of doxycycline (Dox) for 3, 6, 9, 16, and 24 h. Total proteins were collected to determining protein expressions of EMT-related marker genes by a Western blot analysis. (B) Cells were treated with 1 μg/mL of doxycycline for 9 h. Total RNA was collected to determine mRNA expressions of EMT-related marker genes by a real-time RT-PCR analysis. Each value represents the mean ± SE from three independent experiments. * p < 0.05 vs. doxycycline-free cells. VCAM1: vascular cell adhesion molecule 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.