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. 2018 May 8;19(5):1404. doi: 10.3390/ijms19051404

Figure 2.

Figure 2

Stromal vascular fraction (SVF) cellularity and proliferation capacity of SAT- and DAT-derived adipose-derived stem cells (ASC). (A) Cellularity was calculated by correlating the numbers of SVF cells with the amount (g) of processed fat tissue. Data are shown as mean ± SD (n = 6); (B,C) proliferation of SAT and DAT ASC was assessed after culture for 6 days by analysing DNA content (CyQANT) and mitochondrial activity (PrestoBlue). Results are shown as % of DAT (set to 100%) from six patients; (D) representative immunoblot and quantitative assessment of day 3 proliferating ASC from four donors, analysing expression and phosphorylation of protein kinase B (AKT), extracellular signal-regulated kinase ERK 1/2 (p44/42), mammalian target of rapamycin (mTOR), and GAPDH as loading control. Data are shown as mean ± SD. Significance for difference of the means was calculated using a paired t-test (* p-value < 0.05).