Skip to main content
. 2018 May 15;19(5):1466. doi: 10.3390/ijms19051466

Figure 3.

Figure 3

Anti-apoptotic effect of EGCG-5′Glu under SNP-induced apoptosis. (a) EGCG-5′Glu was applied to HaCaT cells for 24 h. Cell viability was tested by MTT assay. (b) EGCG-5′Glu was pre-treated on RAW264.7 cells for 30 min, and SNP (1.5 mM) was added for 24 h. SNP-derived NO was measured by Griess assay. (c) Under SNP treatment, cell viability of HaCaT cells with or without EGCG-5′Glu was identified by MTT assay. (d) Caspase levels of EGCG-5′Glu and SNP-treated HaCaT cells were analyzed by immunoblotting. Antibodies against total or cleaved caspase-3, -8, and -9 and β-actin were used. (e) Phosphorylated levels of PI3K, PDK1, and AKT in EGCG-5′Glu- and SNP-treated HaCaT cells were analyzed by immunoblotting. Antibodies against phospho- or total forms PI3K, PDK1, AKT, and β-actin were used. # p < 0.05 and ## p < 0.01 versus a normal group (untreated group), * p < 0.05 and ** p < 0.01 versus a control group (SNP-treated group).