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. 2018 May 9;19(5):1411. doi: 10.3390/ijms19051411

Figure 1.

Figure 1

Mithramycin A (MitA) inhibits IL-1β-induced expression of matrix-degrading enzymes in mouse primary chondrocytes. (a,b) mRNA expression levels of indicated genes were detected in chondrocytes treated with IL-1β alone or treated with IL-1β and MitA for 24 h. Representative images of RT-PCR (a) and quantification using real-time PCR (b) are shown (n = 5). Values are means ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001); (c) Protein levels of SP1 (cellular) and MMP3/MMP13 (extracellular) were determined by Western blotting.