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. 2018 May 7;19(5):1388. doi: 10.3390/ijms19051388

Figure 5.

Figure 5

AHR inhibitor CH223191 or agonists DIM or TCDD inhibit irregular colony growth in 3D Matrigel cultures. (A) BP1 cells were transfected with pGudLuc and CMV-green vectors. One day later cells were treated with vehicle (DMSO), 10 μM CH223191, 5 μM DIM, or 1 nM TCDD. Normalized pGudLuc luciferase (AHR) activity was assayed 24 h later. Data pooled from 11 experiments are presented as normalized means + SE, * p < 0.05, ** p < 0.01, *** p < 0.001 as compared with the vehicle (0.01% DMSO)-treated controls. (B) BP1 cells were left untreated or treated with vehicle, 10 µM CH223191, 5 µM DIM, or 1 nM TCDD for 24 h. Cells were harvested, counted and plated in Matrigel. Representative images taken at 400× magnification on days 3 and 5 from three independent experiments, with duplicates in each experiment, are shown.