Figure 2.

Developmental effects of lanthionine and/or GSH in zebrafish embryos at 72 hpf. (a–f) shows phenotypes of intact (a) and treated embryos (b,c,e,f), where the survival rates are indicated as percentage of still alive embryos at the end of treatment, compared to the initial number, in each group. Both frontal (c) and lateral (d) view is reported for 1 µM Lan treated embryos; panels (a,b,d–f) depict lateral views. Fluorescence microscope pictures (g–k) represents embryos hearts stained with anti-myosin antibodies (MF20 and S46) using IHC method (whole heart is stained in red and atrium in green). The size proportions of ventricle and atrium (V/A) are indicated in each heart image. Lan, lanthionine; CTRL, control (no treatment).