Figure 5.

Molecular characterization of developmental alterations in zebrafish embryos. Analyses were performed in 105 hpf embryos treated with 0.3 µM lanthionine, 100 µM GSH or with both. (a) WB analysis of protein abundance in whole zebrafish embryos. 15 µg of total proteins were loaded; alpha tubulin (αTub); loading control; (b) Relative quantitation was expressed as percentage of band intensity, normalized to αTub and compared to the samples extracted from untreated control embryos (100%, dashed line). Protein bands were quantitated using ImageJ (National Institutes of Health). The columns represent the mean and error bars indicate the SD of band intensity from two independent experiments in triplicate; (c) Fold changes of AKT, Nrf2a, CBSb and CSE mRNA transcripts in 72 h of 0.3 μM lanthionine treatment (gray bars) in D. rerio embryos compared to untreated controls those indicated as dashed baseline. In (b) data are depicted as mean ± SEM. In both, (b) and (c), p value versus untreated controls; * p < 0.05, ** p < 0.01, *** p < 0.001, while p value versus lanthionine treated embryos; + p < 0.05, ++ p < 0.01, +++ p < 0.001 (according to Student’s t-test). Lan, lanthionine; CTRL, control (no treatment).