Table 1.

The preferred nucleotides at the bleomycin cleavage site.

Study	Type of Break	Preferred Individual Nucleotides						Consensus Sequence from the Individual Nucleotide Data	Consensus Sequence from Complete Sequence Data
Position		−3	−2	−1	0*	+1	+2
Early 32P-end-label experiments	SSB		T	G	T			5′-TGT*
Random DNA sequence	SSB		T	G	T	A		5′-TGT*A	5′-TGT*A
Systematically altered RTGTAY clone	SSB	C > T	C = T	G	T	A	T = A	5′-YYGT*AW
Purified DNA genome-wide preferred nucleotide (50k)	DSB	ns	T	G	T	A	T > A	5′-TGT*AW	5′-TGT*AT
Cellular DNA genome-wide preferred nucleotide (50k)	DSB	G	T	G	T	A	ns	5′-GTGT*A	5′-RTGT*AY

The preferred nucleotides at the bleomycin cleavage site are shown from the −3 to the +2 positions. Only the most highly preferred nucleotides are shown. The preferred nucleotides from the genome-wide data for cellular and purified human DNA are depicted for the top 50,000 cleavage sites. Note that the individual nucleotide analysis at the bleomycin cleavage site (second method), is shown in this Table except for the column on the right where the complete sequence (first method) data is shown. SSB—single-strand break; DSB—double-strand break; ns—not significant. The * indicates the nucleotide at position 0 that is cleaved and destroyed during the reaction.