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. 2018 May 6;19(5):1381. doi: 10.3390/ijms19051381

Figure 1.

Figure 1

Identification and characterization of Fab. (A) The phage antibody expression vector pComb3XSS; (B) PCR amplification of anti-DOPs Fab. (M: DL2000 marker (bp); lane 1: PCR product of κ chain; lane 2: PCR product of the Fd fragment); (C) specificity characterization of seven positive clones with phage-ELISA for anti-DOPs Fab fragment (n = 3); (D) expression of soluble DOPs Fab via SDS-PAGE and Western blot detection, lane 1: protein marker; lane 2: total protein of E. coli TOP 10F’; lane 3: the DOPs-Fab fragment; (E) ELISA standard curve of hapten 1 based on the anti-DOPs Fab fragment (n = 3).