Figure 2.
Target prediction and processes of screening for anti-Ebola microRNAs (miRNAs). (a) Potential target prediction of miRNAs. Interactions between mRNAs and miRNAs were predicted by the online tool RNAhybrid. We selected the seven miRNAs with the highest binding energy for rapid screening for anti-Ebola miRNAs. (b) The whole process of preliminary screening for miRNAs with inhibitory effects. The screening process mainly comprised miRNA expression by plasmids, trVLP infection, and TCID50 detection of the trVLPs in cell culture supernatants.