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. 2018 May 15;19(5):1464. doi: 10.3390/ijms19051464

Figure 3.

Figure 3

Assessing the cell apoptosis ratios of U937 cells treated under various conditions with 0.1% DMSO medium alone (control), BL, HHT, and BL-HHT treatments. The BL treated group is incubated to 24 h after 2 h irradiation, the HHT group is incubated for 24 h without irradiation, and the BL-HHT group is incubated to 24 h after 2 h irradiation. After incubation for 24 h, the apoptosis ratios are detected by flow cytometry. The identification of the fluorochromes are measured and analyzed by FACScan flow cytometry for Annexin V–FITC and PI. (a) The apoptosis ratios are calculated by the Cell Quest software (Becton Dickinson). FACS analysis indicated that the total apoptosis ratios include apparent early apoptosis (lower right (LR) quadrant) and late apoptosis (upper right (UR)); (b) Annexin V–FITC and PI staining of U937 cells detected by fluorescence light microscopy (magnification 40×) after treatment with various groups. The green and red fluorescence represent the early apoptosis cells and late apoptosis, respectively.