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. 2018 May 15;19(5):1464. doi: 10.3390/ijms19051464

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Variations of the apoptosis-related proteins in U937 cells after treatments by BL, HHT, and BL-HHT. The BL-treated group is incubated for 24 h after 2 h irradiation, the HHT group is incubated for 24 h without irradiation, and the BL-0.05 μg/mL HHT group is incubated for 24 h after 2 h irradiation. (a) The activity of caspase-3, (b) caspase-9 are measured using protein activity assay; (c) The cleaved caspase-9, cleaved caspase-3, and cleaved-PARP are determined via immunoblotting assay. β-Actin is used as a loading control. Bars of means ± SD are obtained from three independent cell culture experiments. * p < 0.05 vs. control group.