Figure 5.
Expression of CXCR4 by Western blotting and measurement of reactive oxygen species (ROS) by H2DCFDA using fluorescence intensity. (A) Different IRB concentrations induced different expression levels of CXCR4. At a concentration of 15 μg/mL, CXCR4 RNA was increased by approximately 2-fold (n = 8, *: p ≤ 0.05, ***: p ≤ 0.005). (B) Different IRB concentrations and IRB incubation time induced changes in ROS levels. After 3 h, 20 μg/mL IRBs clearly generated ROS compared to 0 μg/mL. After 24 h, 15 μg/mL induced a nearly 2-fold increase in ROS levels compared to in the control and 20 μg/mL induced a more than 2-fold increase in ROS levels compared to in the control. H2DCFDA and IRBs were added to each well at the same time (n = 9, *: p ≤ 0.05, ***: p ≤ 0.005).