Table 3.
Dicarbonyl and AGE levels in the plasma and central nervous system of full body Glo-1 overexpression mice and wild type littermates after EAE.
| Substrate | Product | Wild Type n = 4 | Glo-1 Overexpression n = 5 | p-Value |
|---|---|---|---|---|
| Plasma | ||||
| MGO (nmol/L) | 1837 ± 578 | 3279 ± 432 | 0.08 | |
| GO (nmol/L) | 1047 ± 44 | 1477 ± 339 | 0.30 | |
| 3DG (nmol/L) | 1690 ± 172 | 2125 ± 167 | 0.11 | |
| Protein bound CML (nmol/mmol Lysine) | 25.9 ± 0. 8 | 26.9 ± 2 | 0.64 | |
| Protein bound CEL (nmol/mmol Lysine) | 6.2 ± 1 | 8.8 ± 0.8 | 0.08 | |
| Protein bound MG-H1 (nmol/mmol Lysine) | 307.9 ± 51 | 274.0 ± 15 | 0.50 | |
| Free CML (nmol/L) | 251.1 ± 28 | 790.4 ± 523 | 0.39 | |
| Free CEL (nmol/L) | 72.7 ± 15 | 330.4 ± 250 | 0.39 | |
| Free MG-H1 (nmol/L) | 64.0 ± 8 | 169.1 ± 114 | 0.44 | |
| Spinal cord | ||||
| MGO (nmol/L) | 3865 ± 500 | 3382 ± 184 | 0.35 | |
| GO (nmol/L) | 2794 ± 630 | 2517 ± 220 | 0.66 | |
| Protein bound CML (nmol/mmol Lysine) | 108.4 ± 4 | 102.3 ± 8 | 0.54 | |
| Protein bound CEL (nmol/mmol Lysine) | 41.9 ± 6 | 46.5 ± 9 | 0.69 | |
| Protein bound MG-H1 (nmol/mmol Lysine) | 270.7 ± 25 | 305.4 ± 47 | 0.57 | |
| Free CML (nmol/L) | 87.3 ± 9 | 92.9 ± 7 | 0.64 | |
| Free CEL (nmol/L) | 17.1 ± 0.8 | 16.4 ± 0.5 | 0.53 | |
| Free MG-H1 (nmol/L) | 4.4 ± 0. 8 | 3.7 ± 0.1 | 0.32 | |
| Glo-1 activity (nmol/mg/min) | 66.6 ± 8 | 367.8 ± 6 | <0.0001 | |
| Brain | ||||
| MGO (nmol/L) | 2906 ± 309 | 2836 ± 127 | 0.83 | |
| GO (nmol/L) | 4086 ± 718 | 4291 ± 354 | 0.79 | |
| Protein bound CML (nmol/mmol Lysine) | 63.9 ± 4 | 57.8 ± 7 | 0.50 | |
| Protein bound CEL (nmol/mmol Lysine) | 40.6 ± 6 | 37.0 ± 2 | 0.55 | |
| Protein bound MG-H1 (nmol/mmol Lysine) | 177.3 ± 16 | 179.4 ± 13 | 0.92 | |
| Free CML (nmol/L) | 52.0 ± 5 | 51.2 ± 3 | 0.88 | |
| Free CEL (nmol/L) | 25.4 ± 2 | 25.4 ± 1 | 0.97 | |
| Free MG-H1 (nmol/L) | 3.3 ± 0.1 | 3.4 ± 0.2 | 0.69 | |
| Glo-1 activity (nmol/mg/min) | 50.1 ± 2 | 296.1 ± 11 | <0.0001 |
Data presented as mean ± SEM and analyzed using unpaired t-test.