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. 2018 May 7;19(5):1390. doi: 10.3390/ijms19051390

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Time-dependent effect on relative sphingosine 1-phosphate (A) and sphinganine 1-phosphate (B) levels in human blood samples. Whole blood samples (EDTA as anticoagulant or serum) were incubated at +22 °C for the indicated times. Thereafter, plasma and serum were isolated as described in the Material and Methods section. Plasma and serum samples without storage time served as the respective controls (0 h; 100%). After final preparation, all samples were stored at −80 °C until LC-MS/MS analysis. Data are shown as the mean (in %) ± SD (n = 4). * p < 0.05, *** p < 0.001.