Table 3.
Temperature-dependent effect on relative sphingolipid levels in human blood samples.
| 0 h | 4 h | ||
|---|---|---|---|
| - | +4 °C | +22 °C | |
| Heparin | |||
| C16Cer | 100 ± 34.5 | 88.3 ± 41.6 | 91.1 ± 23.5 |
| C18Cer | 100 ± 42.7 | 103 ± 44.4 | 120 ± 34.9 * |
| C20Cer | 100 ± 40.6 | 106 ± 46.5 | 91.5 ± 35.0 |
| C24Cer | 100 ± 23.9 | 102 ± 22.8 | 107 ± 25.5 |
| C24:1Cer | 100 ± 26.5 | 95.7 ± 17.8 | 105 ± 32.1 |
| C24dhCer | 100 ± 37.7 | 94.1 ± 26.1 | 94.6 ± 35.6 |
| C24:1dhCer | 100 ± 27.7 | 91.2 ± 24.5 | 100 ± 34.5 |
| EDTA | |||
| C16Cer | 100 ± 23.0 | 107 ± 38.6 | 91.9 ± 24.3 |
| C18Cer | 100 ± 49.1 | 99.1 ± 44.4 | 110 ± 44.1 |
| C20Cer | 100 ± 66.8 | 90.7 ± 32.2 | 102 ± 43.4 |
| C24Cer | 100 ± 25.9 | 102 ± 24.9 | 105 ± 25.8 |
| C24:1Cer | 100 ± 32.0 | 101 ± 25.7 | 106 ± 25.5 |
| C24dhCer | 100 ± 28.9 | 117 ± 36.2 | 98.1 ± 21.9 |
| C24:1dhCer | 100 ± 32.2 | 94.5 ± 23.5 | 103 ± 25.9 |
| Citrate | |||
| C16Cer | 100 ± 29.2 | 110 ± 21.4 | 102 ± 15.4 |
| C18Cer | 100 ± 50.1 | 104 ± 37.3 | 92.3 ± 37.6 |
| C20Cer | 100 ± 51.7 | 98.9 ± 47.5 | 101 ± 41.3 |
| C24Cer | 100 ± 26.0 | 105 ± 24.4 | 104 ± 24.4 |
| C24:1Cer | 100 ± 19.8 | 105 ± 16.7 | 99.5 ± 24.3 |
| C24dhCer | 100 ± 35.9 | 106 ± 26.8 | 117 ± 38.8 |
| C24:1dhCer | 100 ± 26.6 | 102 ± 18.2 | 100 ± 29.3 |
| Serum | |||
| C16Cer | 100 ± 45.4 | 106 ± 46.5 | 116 ± 43.3 |
| C18Cer | 100 ± 42.9 | 102 ± 43.9 | 101 ± 51.1 |
| C20Cer | 100 ± 40.8 | 98.1 ± 34.9 | 93.0 ± 34.2 |
| C24Cer | 100 ± 26.0 | 99.8 ± 21.6 | 99.9 ± 28.1 |
| C24:1Cer | 100 ± 22.3 | 100 ± 13.2 | 109 ± 36.9 |
| C24dhCer | 100 ± 39.4 | 102 ± 24.6 | 107 ± 50.8 |
| C24:1dhCer | 100 ± 31.6 | 90.8 ± 14.0 | 104 ± 34.1 |
Mean (in %) ± SD; n = 4; Repeated ANOVA (Bonferroni post test); * p < 0.05, compared to 0 h.