Table 6.
Effect of sample preparation on relative sphingolipid levels in human blood samples.
| Centrifugation | Freezing | |||||
|---|---|---|---|---|---|---|
| 550× g | 5800× g | 16,200× g | Snap | −20 °C | −80 °C | |
| C16Cer | 100 ± 15.9 | 95.2 ± 17.5 | 93.6 ± 22.8 | 100 ± 36.0 | 112 ± 22.6 | 105 ± 23.0 |
| C18Cer | 100 ± 33.9 | 105 ± 50.0 | 96.7 ± 42.4 | 100 ± 50.2 | 108 ± 43.1 | 111 ± 41.1 |
| C20Cer | 100 ± 35.7 | 94.1 ± 48.3 | 116 ± 4.92 | 100 ± 59.5 | 104 ± 38.0 | 104 ± 52.8 |
| C24Cer | 100 ± 19.7 | 97.2 ± 24.4 | 92.8 ± 25.1 | 100 ± 37.0 | 101 ± 20.0 | 99.7 ± 24.1 |
| C24:1Cer | 100 ± 32.9 | 90.9 ± 25.9 | 89.9 ± 24.4 | 100 ± 36.4 | 103 ± 24.2 | 102 ± 29.0 |
| C24dhCer | 100 ± 17.0 | 92.6 ± 23.6 | 87.7 ± 24.1 | 100 ± 31.1 | 103 ± 21.9 | 103 ± 25.7 |
| C24:1dhCer | 100 ± 11.4 | 93.8 ± 13.2 | 91.0 ± 15.8 | 100 ± 22.1 | 103 ± 17.4 | 101 ± 14.0 |
Mean (in %) ± SD; EDTA; n = 4; Repeated ANOVA (Bonferroni post test).