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. 2018 May 16;19(5):1485. doi: 10.3390/ijms19051485

Figure 4.

Figure 4

Expression of fatty acid metabolism genes is decreased in csPIMT−/− hearts. (A) Quantification of mRNA levels of Lcad (Long chain acly-CoA dehydrogenase), Mcad (medium-chain acyl-CoA dehydrogenase), Scad (short-chain acyl-CoA dehydrogenase) and Ucp3 (uncoupling protein 3) genes. * p < 0.05, ** p < 0.01, NS: not significant; (B) Western blot showing protein levels of LCAD (47 Kda), MCAD (46 Kda), SCAD (44 Kda) and l-PBE (78 Kda; Enoyl-CoA hydratase /l-3-hydroxyacyl-CoA dehydrogenase). Percent reduction (KO vs. WT) for LCAD, 40%; MCAD, 39%; SCAD, 45%, and l-PBE, 27% for 6-month time point. Each group was analyzed using 5 different mice and the values were expressed as the mean ± SD; (C) Western blot showing the protein levels of DRP1 (78 Kda) and PINK1 (60 Kda). Details of same as in (A). See Materials and Methods for antibody sources and dilutions.