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. 2018 May 16;19(5):1485. doi: 10.3390/ijms19051485

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Myocardial fibrosis in csPIMT^−/− mouse hearts. (A) Images of Masson trichrome staining patterns of a representative PIMT^fl/fl and csPIMT^−/− hearts of 2, 4 and 6 months are shown (magnification 400×). Note the intensely stained (blue color) interstitial fibrous strands in 6-month-old csPIMT^−/− hearts; (B) Quantification of mRNA levels for Tgfβ2, Ctgf and Col9a2 in csPIMT^fl/fl and csPIMT^−/− hearts of 2, 4 and 6 months of age. mRNA levels were quantified by RT-qPCR assays. Each group was analyzed using 5 different mice and the values were expressed as the mean ± SD. * p < 0.05, ** p < 0.01.