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. 2018 May 4;19(5):1364. doi: 10.3390/ijms19051364

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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S- or G2/M-phase synchronization at DPI-1 enhances cell-cycle exit in GMT-reprogrammed iCMs. (A) At DPI-1, MEFs were synchronized at G1, G0/G1, G1/S, or G2/M-phase by lovastatin, serum-free media, thymidine, or nocodazole, respectively. Representative pictures showing GMT-reprogrammed MEFs at DPI-10 with or without (Control) cell-cycle synchronization. Scale bars indicate 100 µm. (B) Representative FACS plots of reprogrammed αMHC-GFP⁺ iCMs at DPI-10. (C) The effect of G1-, G1/S-, or G2/M-phase synchronization on GMT-iCMs (n = 10), including the percentage (upper panel) and absolute number (lower panel) of αMHC-GFP⁺ iCMs at DPI-10. (D) The effect of S-phase synchronization by aphidicolin, hydroxyurea, or L-mimosine on GMT-iCMs (n = 5) at DPI-7. (E) The percentage of EdU⁺ cells in αMHC-GFP⁺ iCM-population at DPI-7 with or without cell-cycle synchronization at DPI-1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. GMT group.