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. 2018 Apr 26;19(5):1297. doi: 10.3390/ijms19051297

Table 3.

Primers and associated annealing temperatures.

Gene/lncRNA Primer Sequence Temp Source
EGFR-AS1 F: 5′-CTTTGCGATCTGCACACACC-3′ 62 This study
R: 5′-GAAGCCTACGTGATGGCCAG-3′
PCAT6 F: 5′-CCCTAGATACACCCGCCTGGT-3′ 64 This study
R: 5′-ACATTCCAGGGCACCGAGAG-3′
ZEB2-AS1 F: 5′-GAGAGAGACGAGAGACCCTGAA-3′ 60 This Study
R: 5′-AAATTCATCATGCACACACCC-3′
KDM5B (JARID1B) F: 5′-GCTACCCCCTCCAGCTACTCAGA-3′ 62 This study
R: 5′-TCCTCCTCGACTTCCTCCTCATC-3′
18S rRNA F: 5′-GATGGGCGGCGGAAAATAG-3′ 60 [149]
R: 5′-GCGTGGATTCTGCATAATGGT-3′