Figure 3.

High fat diet induced sarcopenia that was fully reverted by somatotropic hormone supplementation. (A) Muscle histological sections with wheat germ agglutinin (WGA) fluorescence staining, (B) muscle fibre diameter distribution as determined by measure of the minimal feret’s diameter, (C) analysis of three muscle fibre size ranges. Thick (>56 Lm), medium size (30–55 Lm), and thin (<30 Lm) muscle fibres are shown. (D,E) electrophysiological analysis of TA muscle strength from both groups including twitch and tetanus contractions. (F) Body strength in vivo. * p < 0.05; ** p < 0.01; N.D., not detectable.