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. 2018 May 22;19(5):1537. doi: 10.3390/ijms19051537

Figure 2.

Figure 2

Effect of ethanol on CBS and CSE protein expression. A representative immunoblot image detecting CSE and ACTIN protein (upper panel) and its quantification (bottom panel) from Control and E-treated PCNs for indicated time points (hours) (n = 4) (A); Immunodetection of CBS and ACTIN protein in Control and E-treated PCNs measured by Western blotting (upper panel) and the corresponding quantification of CBS protein levels normalized to the reference protein, ACTIN (bottom panel) (n = 6) (B); Western blot image of CSE and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein in fetal brain cortices obtained from Control or in utero E-exposed pregnant dams (upper panel) and the image densities of CSE relative to GAPDH (bottom panel) (n = 4) (C); Representative Western blots demonstrating the expression of CBS protein, with GAPDH as a loading control in fetal brain cortices of control and in utero alcohol-exposed pregnant rats (upper panel) and the quantification of immunoblots of CBS normalized to GAPDH (bottom panel) (n = 4) (D). Data are represented as mean ± SEM. * p < 0.05 was considered significant for ethanol.