Figure 3.

Effect of ethanol on the expression of Cse and Cbs mRNA. PCNs were treated with E (4 mg/mL) for the periods indicated and real-time qRT-PCR analysis for Cse transcript expression was performed. The fold change expression of Cse was determined by normalizing with the expression of a housekeeping gene, Gapdh (n = 4) (A); Real-time qRT-PCR based mRNA expression of Cbs gene relative to Gapdh from control and E-treated PCNs (n = 4) (B); Fetal brain cortices obtained from 2-day binge administration of iso-caloric dextrose and ethanol (as in Materials and Methods) was subjected to real-time qRT-PCR analysis for Cse mRNA expression (n = 5) (C). Real-time qRT-PCR analysis of Cbs gene relative to Gapdh from fetal brain cortices obtained from 2-day iso-caloric dextrose and ethanol-administered pregnant dams (as in Materials and Methods) (n = 5) (D). Values represent the mean ± SEM. * p < 0.05 was considered significant for ethanol alone.