Figure 2.

Effects of Mg withdrawal on cell cycle distribution and intracellular Mg concentration in adipose-derived mesenchymal stem cells (AD-MSCs). (A) Cell cycle distribution of AD-MSCs cultured in reprogramming medium (RM) or control medium (CM) at 5 and 10 days in physiological concentrations of Mg (upper table) or in Mg-deficient medium (lower table). The results are the mean of three experiments, carried out in triplicate. (B) Total Mg concentration was measured in treated (RM 0.1 mM Mg) and untreated (CM 0.1 mM Mg) AD-MSCs after 5 and 10 days in Mg-deficient medium. Measurements were carried out in sonicated sample by using the fluorescent probe DCHQ5.