Figure 3.

TRβ mRNA expression in cerebellar granule cells in the absence, Glia−, or presence of glial cells, Glia+; treated with zearalenone (ZEN), and/or triiodo-thyronine (T3), thyroxine (T4) and 17β-estradiol (E2) for 6 h. (a) Relative expression level of the TRβ gene was analyzed by qRT-PCR and normalized to the average of the control gene β-actin. Shown p-values were calculated: Glia+ compared to Glia− (**) p < 0.01, (***) p < 0.001 in each treatment group; (b) Relative expression of TRβ mRNA in non-treated controls normalized to β-actin (p-value not shown). The data shown here are the mean ± SD of at least three independent experiments (n = 6 per treatment).