Fig 4. Dynamics of MNP subsets in the tongue during acute OPC.

(A) Flow cytometric analysis of CD11c⁺MHCII⁺ MNP subsets in tongue of naïve and infected WT mice for the expression of Ly6C. Pre-gating is on CD45⁺ cells. Numbers in the histogram indicate MFI (median) of Ly6C expression. (B) Summary plot with absolute numbers of the CD11c⁺MHCII⁺ MNP subsets according to the analysis in A in naïve and infected tongue of WT animals. Each symbol represents one animal, the mean of each group is indicated. Graphs show pooled data from two independent experiments. (C) Analysis of CD11c⁺MHCII⁺CD11b^highLangerin^- cells for Ly6C and CCR2 in the tongue of naïve and infected animals. An overlay of CD11c⁺MHCII⁺CD11b⁺Langerin^-Ly6C⁺CCR2⁺ inflammatory monocytes (blue) onto all CD45⁺ cells (red) derived from naïve and infected WT mice is shown. Representative FACS plots are from one out of two independent experiments are shown. (*p<0.05; ****p<0.0001). See also S5 Fig.